Journal: Molecules

Article Title: In Vitro Validation of Phosphorodiamidate Morpholino Oligomers

doi: 10.3390/molecules24162922

Figure Lengend Snippet: ( A ) RT-PCR products of ITGA4 transcript amplified from human fibroblasts nucleofected with ASO 2 at 50 µM concentration in the cuvette using the P3 nucleofection kit and CA-137 program and various cell densities. The number of cells nucleofected is shown above the gel. Shown are the average percentages of exon skipping from three biological replicates represented as a bar graph next to the gel image. UT: Untreated. Error bar; SEM. ( B ) RT-PCR products of SMN transcript from Huh7 cells nucleofected with ASO 3 at 50 µM concentration in the cuvette using P3 kit, and CA-137 or Ex-147 program. Shown are the average percentages of exon skipping using CA-137 program from three biological replicates represented as a bar graph next to the gel image. UT: Untreated. Error bar; SEM.

Article Snippet: All nucleofections were performed using the 4D-Nucleofector™ X Unit and P3 kit (Lonza, Mt Waverley, VIC, Australia) with the concentration of PMO set at 50 µM in the cuvette (volume of 1 µL 1 mM PMO in 20 µL cuvette volume), unless otherwise stated.

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Concentration Assay

Journal: Molecules

Article Title: In Vitro Validation of Phosphorodiamidate Morpholino Oligomers

doi: 10.3390/molecules24162922

Figure Lengend Snippet: Optimal nucleofection programs for the P3 kit and the cell numbers tested in specified cell lines.

Article Snippet: All nucleofections were performed using the 4D-Nucleofector™ X Unit and P3 kit (Lonza, Mt Waverley, VIC, Australia) with the concentration of PMO set at 50 µM in the cuvette (volume of 1 µL 1 mM PMO in 20 µL cuvette volume), unless otherwise stated.

Techniques: